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KMID : 1140120090140020099
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Cancer Prevention Research
2009 Volume.14 No. 2 p.99 ~ p.103
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Regulation of AMP-activated Kinase and Cyclooxygenase-2 by Estrogen in Non-cancerous, Tumor-promoter Stimulated Non-cancerous or Breast Cancer Cells
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Park Song-Yi
Lee Yun-Kyoung
Kim Young-Min
Lee Won-Sup
Park Ock Jin
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Abstract
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Estrogen has been suggested as a promoter of breast cancer for its cell proliferative effect and antiapoptotic properties, and moreover, recent epidemiological data suggest that females are at higher risk for lung cancers than males, and it is attributed to the greater exposure to the genotoxicity of estrogen metabolites in the form of hydroxyestradiols. Our previous studies have revealed that AMP-activated protein kinase (AMPK) activation is strongly related to the inhibition of cancer cell growth. We examined the regulation of AMPK and cyclooxygenase-2 (COX-2) by exogenous estrogen in non-cancerous normal cells, TPA (12-O-tetradecanoylphorbol-13-acetate)-treated normal cells and MCF-7 breast cancer cells to elucidate the effects of estrogen treatment on AMPK and COX-2. The exogenous estrogen strongly stimulated COX-2 expression in TPA treated cox-2 ＋/＋ cells and MCF-7 cells, but such stimulation was not observed with non-TPA treated normal cells. Estrogen activated AMPK in all three kinds of cells, and in TPA-treated and cancerous cells the activation of AMPK with estrogen could not abolish COX-2 expression. These results indicate that the cell type is an important defining factor of AMPK and COX-2 regulation by estrogen, and this may have some significance to unravel the possible link between estrogen and cancers.
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KEYWORD
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Estrogen, TPA treatment, AMPK-COX-2 pathway, Carcinogenesis, MCF-7 breast cancer cells
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